|
|
|
|
|
Allen-Hoffmann, B. Lynn
B. Lynn Allen-Hoffmann, PhD
Professor
Associate PI on METC NIH/NIEHS Pre/Post Doctoral Training Grant
Research Area: Epithelial Differentiation and Carcinogenesis. Discovered and characterized mechanism of Ah receptor activations affected by disruption of cell adhesion. Role of Ah rceptor and other bHLH-PAS family members in development and xenobiotic induced pathogenesis of human stratified squamous epithelia. Tissue engineered human models for toxicity testing.
Home Dept: Department of Pathology & Laboratory Medicine, School of Medicine and Public Health
Affiliated Depts: Molecular and Environmental Toxicology
Address
5605 Medical Sciences Center
1300 University Avenue
Madison, WI 53706
Phone: 608/262-2884 - Email
Patents
2006 - Keratinocytes Expressing Exogenous Angiogenic Growth Factors
2005 - Method and composition for skin grafts
2005 - Skin substitutes with improved barrier function
2005 - Skin substitutes and uses thereof
2002 - Immortalized human keratinocyte cell line
Company
Stratatech Corporation - Founded in 2000 - A regenerative medicine company focused on the development of cell-based, tissue-engineered products for wound care. The keystone of Stratatech's approach is a proprietary human skin cell line that when properly cultured forms a fully stratified multi-layered human tissue with the physical strength and biological characteristics of intact human skin. This biologically active tissue is suitable for transplantation on patients using traditional surgical suturing techniques. Stratatech is developing new products that have been genetically enhanced to over-express natural, human wound-healing factors that reduce infection, improve blood flow to the wound, and increase the rate of healing with reduced scarring. The company has generated the first engineered human tissue in the history of the state and is the first company founded by a woman scientist at the University of Wisconsin. Stratatech has assembled a team of experienced research, production, clinical, and regulatory personnel to facilitate the discovery, development, and commercialization of their products.
Research
Our laboratory is investigating the role of catenin-mediated signal transduction in aryl hydrocarbon receptor (AhR) function using a variety of state of the art techniques including somatic cell knockouts and inhibitory RNA technology. We have been able to answer questions regarding AhR and Arnt signaling in tissue engineered models of human skin and is dissecting the proximal tissue interactions important for AhR ligand toxicity in human stratified squamous epithelia. We have initiated studies using intact human skin samples to confirm and extend findings in tissue engineered models. Our group investigates the effects of physiological stressors, such as hypoxia, and environmental stressors, such as environmental toxins, and wounding on tissue regeneration and function. The laboratory is using both human epidermal progenitors and human embryonic stem cells to generate relevant human-specific models of tissue and developmental pathophysiology.
Representative Publications
- Slavik MA, Allen-Hoffmann BL, Liu BY, Alexander CM. Wnt signaling induces differentiation of progenitor cells in organotypic keratinocyte cultures. BMC Dev Biol. 2007 17;7:9.
- Ji L, Allen-Hoffmann BL, de Pablo JJ, Palecek SP.
Generation and differentiation of human embryonic stem cell-derived keratinocyte precursors. Tissue Eng. 2006 12(4):665-79.
- Loertscher JA, Lin TM, Peterson RE, Allen-Hoffmann BL.
In utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin causes accelerated terminal differentiation in fetal mouse skin. Toxicol Sci. 2002 68(2):465-72.
- Loertscher JA, Sattler CA, Allen-Hoffmann BL. 2,3,7,8-Tetrachlorodibenzo-p-dioxin alters the differentiation pattern of human keratinocytes in organotypic culture. Toxicol Appl Pharmacol. 2001 175(2):121-9.
- Loertscher JA, Sadek CS, Allen-Hoffmann BL. Treatment of normal human keratinocytes with 2,3,7,8-tetrachlorodibenzo-p-dioxin causes a reduction in cell number, but no increase in apoptosis. Toxicol Appl Pharmacol. 2001 175(2):114-20.
Check PubMed for other publications by B. Lynn Allen-Hoffmann
|
|
|
|
