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Bugni, Tim
Tim S. Bugni, PhD
Assistant Professor
Research Area -
Home Dept - Pharmacy
Affiliated Depts - Molecular & Environmenal Toxicology
Address
School of Pharmacy
6111 Rennebohm Hall
777 Highland Ave.
Madison, WI 53705-2222
Phone: (608) 263-2519 - Email
Professional Memberships- Member of the American Society of Pharmacognosy
- Member of the American Chemical Society
- Member of the International Symbiosis Society
Research Summary
The overall goal of the Bugni lab is to discover new biologically active small molecules as potential therapeutics. Small molecules from nature (natural products) have been a rich source of therapeutics, particularly as anticancer agents and antibiotics. The laboratory investigates marine invertebrate associated bacteria to understand bacterial diversity within whole animal tissues and to explore the therapeutic potential of bacterial produced natural products.Marine invertebrate-associated bacteria: Many natural products isolated from whole marine animals, such as sponges and ascidians, are likely produced by symbiotic bacteria. In collaboration with Professor Eric Schmidt (University of Utah), we are developing new methods to evaluate total bacterial diversity and molecular diversity of whole animals. Additionally, we are developing analytical approaches to understand natural product diversity from cultivable bacteria with the goal of rapidly identifying the most promising strains for drug discovery.
Isolation and structure determination of natural products: With the goal of discovering new natural product scaffolds with therapeutic potential, we use a combination of assays to detect biological activity and chemoinformatics to guide our discoveries. In terms of chemoinformatics, we are applying metabolomics tools for the analysis of large numbers of bacterial produced natural products. Additionally, the laboratory uses state-of-the-art high-resolution mass spectrometry as well as ultra-sensitive NMR spectrometers to rapidly characterize natural products on nanomole to picomole scales.
Integrating natural products with high-throughput screening: High-throughput screening (HTS) has the potential to test large numbers of compounds in a single day (>25,000). Most HTS platforms are not compatible with many aspects of natural products. By employing a high level of automation in the laboratory, we are developing methods to produce high quality natural products libraries and associated tools for prioritization of hits.
Recent Publications- Hou Y, Braun D, Michel C, Wyche TP, Adnani N, Bugni TS* Secondary metabolomics using ultra-high performance liquid chromatography coupled with ultra-high resolution ESI-Q-TOF mass spectrometry and principal component analysis as a broadly useful tool for rapid analysis of microbial natural products. Anal Chem 2011 [Manuscript Submitted]
- Hu K, Wyche TP, Bugni TS, Markley JL Selective quantification by 2D HSQC0 spectroscopy of thiocoraline in an extract from a sponge-derived Verrucosispora sp. J Nat Prod 2011; Sep 16. [Epub ahead of print]
- Wyche TP, Hou Y, Braun D, Cohen HC, Xiong MP, Bugni TS* First natural analogs of the cytotoxic thiodepsipeptide thiocoraline A from a marine Verrucosispora sp. J Org Chem 2011; 76: 6542-6547
- Pimentel-Elardo SM, Buback V, Gulder TAM, Bugni TS, Reppart J, Bringmann G, Ireland CM, Schirmeister T, Hetschel U New tetromycin derivatives with anti-trypanosomal and Protease Inhibitory
activities. Marine Drugs 2011, 9, 1682-1697 - Luo Y, Li Y, Ju J, Yuan Q, Peters NR, Hoffman FM, Huang SX, Bugni TS, Rajski S, Osada H, Shen B. Functional characterization of TtnD and TtnF, unveiling new insights into tautomycetin biosynthesis. J Am Chem Soc 2010; 132: 6663-6671
- Coombs GS, Yu J, Canning CA, Veltri CA, Covey TM, Cheong JK, Utomo V, Banerjee N, Zhang ZH, Jadulco RC, Concepcion GP, Bugni TS, Harper MK, Mihalek I, Jones CM, Ireland CM, Virshup DM.
WLS-dependent secretion of WNT3A requires Ser209 acylation and vacuolar acidification. J Cell Sci 2010; 123: 3357-3367 - Koch M, Bugni TS, Sondossi M, Ireland CM, Barrows LR. Exocarpic acid inhibits mycolic acid biosynthesis in Mycobacterium tuberculosis. Planta Med 2010; 76, 1678-1682
- Wei X, Bugni TS, Harper MK, Sandoval IT, Manos EJ, Swift J, Van Wagoner RM, Jones DA, Ireland CM. Evaluation of pyridoacridine alkaloids in a zebrafish phenotypic assay. Mar Drugs 2010; 8: 1769
- Pimentel-Elardo SM, Kozytska S, Bugni TS, Ireland CM, Moll H, Hentschel U. Anti-Parasitic compounds from Streptomyces sp. Strains isolated from Mediterranean sponges. Mar Drugs 2010; 8:373-380
Check PubMed for other publications by Tim S. Bugni
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