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- Current Students
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Student Resources
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Student Publications
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Blanke, Kristina
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Boley, Patricia
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Bolterstein, Elyse
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Booth, Clarissa
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Brody, Matthew
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Bultman, JoAnna
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Burns, Felipe
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Desotelle, Josh
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Ding, Lina
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Elmergreen, Tammy
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Hutchinson, John
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Irving, Amy
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Irving, Roy
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Johnson, Brian
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Johnson, Delinda
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Johnson, Shaina
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Jung-Hynes, Brittney
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Kumar, Kartik
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Lee, Sung-Kyoung
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Lorch, Jeff
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Mehta, Vatsal
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Novick, Rachel
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Park, Heesoo
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Pham, Ly
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Poenitzsch, Ashley
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Rhoads, Keelia
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Rufer, Echoleah
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Sand, Jordan
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Schmit, Travis
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Shan, Weihua
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Shanle, Erin
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Shetty, Ameesha
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Syed, Deeba
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Tarapore, Rohinton
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Velasco, Javier
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Wiecinski, Paige
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Wong, Letitia
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Yang, Sarah
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Yu, Min
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Zhao, Yun
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Calkins, Marcus
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Bolterstein, Elyse
 Elyse Bolterstein - Email
PhD Candidate - Started 2004
Rochester Hills, Michigan
Lab of B. Lynn Allen-Hoffmann, PhD
Undergraduate Work
University of Michigan
Bachelor of Science, Resource Ecology & Management (2002)
Memberships
Society of Toxicology - 2008
Funding
National Research Service Award Predoctoral Traineeship (NIEHS Training Grant T32, 02/2005-__/__/09)
Interests/Hobbies
I like to spend a lot of my free time outdoors: biking, running, hiking, browsing the tasty things at the farmer’s market and snowshoeing when the weather gets too nasty to do the other things. My indoor hobbies include reading, cooking and teaching a UW spinning class a couple times a week. Although there are a couple Madison sports I’m completely into (Mallard’s baseball and Badger hockey), I’m a diehard Michigan football fan.
Why I Joined METC
Besides being a well-recognized, highly-reputable program, the aspect that most attracted me to METC was the program’s environmental emphasis. The field of toxicology was a natural match for me to apply my ecological background and interests and therefore it was important that I would have an opportunity to study environmentally relevant compounds. When I first visited Madison, I was particularly impressed with the leadership and influence the students had within the program, as well as the friendly student/faculty interactions, which were uncommon in my visits to other universities. It also didn’t hurt that Madison is a beautiful city to spend my time while working on my degree.
Research Fall 2008
The pluripotent nature and unlimited proliferative ability of human embryonic stem (hES) cells have potential to provide a renewable, consistent cell source, in which to more accurately study toxicity in early human development. However, a relatively small number of published reports study toxicological endpoints and pathways in hES cells. Investigations of responses to xenobiotic exposure in animal models have been wrought with interspecies differences that have complicated data interpretation and therefore cannot be relied upon to effectively model toxic responses in humans.
The aryl hydrocarbon receptor (AhR)/aryl hydrocarbon receptor nuclear translocator (Arnt) pathway is central to xenobiotic toxicity observed in various tissues and critical to proper developmental progression. When exposed in utero to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD), an environmental contaminant and potent AhR/Arnt pathway agonist, rodents exhibit developmental abnormalities such as cleft palate. Futhermore, TCDD toxicity has been shown to result in regenerative growth deformities, possibly due to changes in expression of extracellular matrix remodeling genes and subsequent alterations in epithelial to mesenchymal transition (EMT).
Previous work in our lab has shown that the AhR/Arnt pathway is functional in human embryonic stem (hES) cells in both undifferentiated and differentiated monolayer culture, as well as in highly differentiated embryoid bodies (EBs) derived from hES cells. My current work focuses on examination of TCDD exposure on the differentiation status of hES cells and EBs using PCR analysis. TCDD treatment appears to inhibit differentiation, as evidenced by lower mRNA expression of early differentiation markers in hES cells and greater expression in EBs. Additionally, localization of EMT-related proteins, as visualized through indirect immunofluorescence, suggests that TCDD may enhance progression of EMT in EBs. These findings support use of hES cells as a tool for exploring mechanisms in developmental toxicology.
 Posters
AhR pathway genes are expressed in human embryonic stem cells
Elyse A. Bolterstein1 and B. Lynn Allen-Hoffmann1,2
1Molecular and Environmental Toxicology Center and 2Department of Cellular and Molecular Pathology School of Medicine and Public Health - University of Wisconsin-Madison; Madison, Wisconsin, USA (Presented at 2008-SOT in Seattle, WA and at the 2008 World Stem Cell Summit in Madison, WI)
RNAI - Mechanisms and Tools for Toxic Control in Aspergillus Species. E. A. Bolterstein1 and N. P. Keller1,2
1Molecular and Environmental Toxicology, University of Wisconsin, Madison, WI
2Plant Pathology, University of Wisconsin, Madison, WI (Presented at 2006-SOT in San Diego)
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