Shan, Weihua - Date Last Updated: 09/29/2009

Weihua Shan - Email
PhD Candidate - Started 2005
Native of China
Lab of Terry Oberley, PhD

Undergraduate Work
Shandong Medical University, Jinan, Shandong Province, China
Bachelor of Science, Pharmaceutical (1995)

Honors/Awards
1999 Shandong province Science & Technology Advancement Award.
Shandong Province Government. China

Patent
National patent of preparation of new oral solution to cure of flue by Chinese traditional drugs. 1998, Open number CN1188005A (The State Intellectual Property Office of People’s Republic of China)

New Drug Certificate
New drug certificate of Chaihuang oral solution (1995); Tablet of Shuandan (1998); Capsule of Shuandan (1998); Capsule of Chaihuang (1998) (The State Food and Drugs Administrative of Peoples’ Republic of China)

Research as of April 2006
Prostate cancer (PC) has become the most frequently diagnosed neoplasm and the second leading cause of cancer-related mortality in men, but the mechanism to induce prostate cancer is not well illustrated. Epidemiological, experimental and clinical studies have implicated oxidative stress in the development and progression of prostate cancer, hence, a greater understanding of the molecular events associated with oxidative stress will enhance our ability to understand and devise better chemo-preventive and chemotherapeutic strategies for controlling PC progression. Evidence suggests that reactive oxygen species (ROS) and reactive nitrogen species (RNS) play a significant but paradoxical role acting as a “double edged sword” to regulate cellular processes, and the compartmental modification of the redox state of cysteine residues of certain proteins by ROS and RNS, which is a widespread mechanism in the regulation of protein function, has been proposed to be involved in signaling pathways. Therefore, it is important to understand underlying signaling mechanisms involved in ROS induced effects on prostate cell growth, cell-cycle checkpoints, apoptosis and transcription factors and the role of potential antioxidants on these mechanisms.

My research project will investigate the role of ROS/RNS in human prostate cancer cells as opposed to nonmalignant, immortalized human prostate epithelial cells, as well as the underlying molecular mechanism. First we will characterize and compare these 2 cells lines in aspects of redox status, redox compartmentation, and their influence on cell behaviors and functions. Second, we will compare how the cells behaviors change differently and compartmental oxidation of thiol-disulphide redox couples such as Trx and GSH/GSSG in response to oxidative stress inducing or/and inhibiting agents, try to figure out key components and redox signaling pathway involving in regulation and differentiation of the two cells. Thirdly, MnSOD is a crucial antioxidant enzyme protecting mitochondria against oxidative stress and evidence has indicated the important role of MnSOD in tumor suppression in several cell types, thus we want to determine whether MnSOD overexpression has different effect on cell behavior and cell biochemical feature in both cells, try to understand how overexpression of MnSOD regulate cell redox signaling form mitochondria to the cytoplasm and/or nuclear after transfection with MnSOD.

Publications

Weihua Shan, Ling Zhang, Yanzeng Shi, Ruixue Liang, Lixia Shang and Qinwen Hu. Quantitative Determination of Ganodermic Polysaccharide and Total Saccharide in Lingzhi Fermented Liquid. Lishizheng Medicine and Material Medical Research. 2001, 11(9): 797-798
Weihua Shan, Ling Zhang, Yanzeng Shi, Xingang Xu, Benming Xu and Chunling Li. Determination of the concentration of Cinnamic acid in Lianhong Yuchang Ling. Lishizheng Medicine and Material Medical Research. 1999, 10(5): 330
Weihua Shan, Ling Zhang, Xingang Xu, Yanzeng Shi, and Chunling Li. Effect of Different Alcohol Concentration on Paeonif lo in Content in Mudan Pi. Lishizheng Medicine and Materia Medica Research. 1998, 9(4): 320-321
Ling Zhang, Weihua Shan, Ruixue Liang, Lixia Shang, Qingwen Hu and Yanzeng Shi. Study on Extraction Processes of Black ant. Traditional Chinese Drug Research & Clinical Pharmacology. 2002, 13(2): 116-118
Ling Zhang, Weihua Shan, Ruixue Liang, Yanzeng Shi. Lixia Shang, Wenhao Chen and Qingwen Hu. Study on Extraction Processes in Panax ginseng. Lishizheng Medicine and Material Medical Research. 2001, 11(9): 777-778
Ling Zhang, Weihua Shan, Yanzeng Shi, Xingang Xu and Chunling Li. Study on the processes of solid substance of evaporable oil from Cinnamomum cassia Presl with ā-Cyclodextrin. Chinese Traditional Patent Medicine 1999, 21 (11): 561-563

Date Last Updated: 09/29/2009 webteam@med.wisc.edu